Study on the Extraction Rate of Morphine Before and After Hair Grinding
Xiao Ruisen,
Huang Ping,
Wang Hong,
Li Jinwei
Issue:
Volume 4, Issue 1, March 2018
Pages:
1-4
Received:
3 May 2018
Accepted:
30 May 2018
Published:
19 June 2018
Abstract: 1500 morphine-absorbed hair was collected, and the hair was ground with a quick grinder to extract the morphine component of the hair. The morphine was qualitatively detected by gas chromatography/mass spectrometry (GC/MS). The test results were processed by statistical software and the hair was evaluated. Morphine positive tests results. A total of 1500 morphine users were found to have 1465 morphine-positive (97.7%) in the hair-dried group and 998 morphine-positive (66.5%) in the unpolished hair group. The results were statistically significant (P < 0.005). In the analysis of the influencing factors of the testing method, the sample collected by the sex, age, and hair collection location of the drug addicts had no effect on the test results (P>0.05); the chemical dyeing, regardless of whether the hair was ground or not, showed a significant decrease in morphine positive detection. The test results have an effect (P<0.05). The results of the study showed that morphine was extracted from ground hair and the positive detection of morphine was significantly improved, with a positive rate of 97.67%. This prevented the missed detection in the drug addiction certification work and made the certification test more scientific and accurate.
Abstract: 1500 morphine-absorbed hair was collected, and the hair was ground with a quick grinder to extract the morphine component of the hair. The morphine was qualitatively detected by gas chromatography/mass spectrometry (GC/MS). The test results were processed by statistical software and the hair was evaluated. Morphine positive tests results. A total o...
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Comparative Studies on the Interaction Between the Medicine Small Molecule with Pepsin by Fluorescence Quenching Spectroscopy and Improved Spectroscopy
Hongcai Zhang,
Baosheng Liu,
Xu Cheng,
Jinju Wang
Issue:
Volume 4, Issue 1, March 2018
Pages:
5-9
Received:
4 May 2018
Accepted:
7 June 2018
Published:
28 June 2018
Abstract: The binding mechanism between cefetamet pivoxil (CFP) and pepsin (PEP) at different temperatures (298 K, 303 K, 310 K) was investigated by the classical fluorescence spectroscopy with focus on the fluorescence change of protein, as well as the improved spectroscopy with focus on the fluorescence changes of the resonance light scattering of small molecule drugs. The results showed that the main quenching mode of PEP-CFP was static quenching. The value of n was approximately equal to 1 which indicating that there was only one binding site in the interaction between PEP and CFP and the Hill coefficient was about 1 which indicating that there was no cooperative between the receptor PEP and ligand CFP. The binding constants of the PEP-CFP system obtained by the improved spectroscopic method were two orders of magnitude larger than that of the traditional fluorescence spectroscopy, which showed that the study of the small drug molecule was more practical and reasonable. The rationality of the experimental results obtained was verified by ultraviolet absorption spectroscopy.
Abstract: The binding mechanism between cefetamet pivoxil (CFP) and pepsin (PEP) at different temperatures (298 K, 303 K, 310 K) was investigated by the classical fluorescence spectroscopy with focus on the fluorescence change of protein, as well as the improved spectroscopy with focus on the fluorescence changes of the resonance light scattering of small mo...
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