In Vivo Antiplasmodial Activity of Fractions and Chromatographic Sub Fractionions of Ethanol Extract of Clerodendrum Myricoides Leaves
Gebremichael Gebretsadik,
Yalemtsehay Mekonnen
Issue:
Volume 2, Issue 6, December 2016
Pages:
60-64
Received:
2 June 2016
Accepted:
15 July 2016
Published:
3 November 2016
Abstract: Herbal medicine preparations from medicinal plants to treat malaria are potential source of new anti-malarial drug development. The objective of this study was to evaluate antiplasmodial activity of fractions and sub fractions of the ethanol extract of Clerodendrum myricoides leaves using 4-day suppressive test against Plasmodium berghei in Swiss albino mice. Methanol fraction (MF) and ethyl acetate fraction (EF) obtained from the successive fractionation of ethanol crude extract of C. myricoides showed highest activity with suppression of 77.24% and 65.21% at an oral dose of 300 mg/kg/day respectively. Further, bioactivity guided fractionation (BGF) of ethanol extract provided some fractions which exhibited good antiplasmodial activity. From these, Hexane sub fraction (Hsf) was the most active that induced suppression of parasitaemia by 40.34% and 46.56% at 50 mg/kg and 100 mg/kg doses respectively. Hsf chromatographic sub fractions also induced suppression of parasitaemia by 45.89 and 50.53% at 20 mg/kg and 40 mg/kg of Hsf-5, respectively, and by 38.61 and 44.12% at 20 mg/kg and 40 mg/kg of Hsf-14, respectively. Bioactivity guided fractionation resulted in loss of antiplasmodial activity of the fractions and sub fractions and this leads to the conclusion that the activity of the plant leaves was due to synergistic effect of many active compounds
Abstract: Herbal medicine preparations from medicinal plants to treat malaria are potential source of new anti-malarial drug development. The objective of this study was to evaluate antiplasmodial activity of fractions and sub fractions of the ethanol extract of Clerodendrum myricoides leaves using 4-day suppressive test against Plasmodium berghei in Swiss a...
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Development and Validation of Alternative Analytical Method for Determination of Related Substances of Benzydamine Hydrochloride in Oral Spray by HPLC
Vasyl A. Cherniy,
Svitlana N. Gureeva,
Victoriya A. Georgiyants
Issue:
Volume 2, Issue 6, December 2016
Pages:
65-73
Received:
20 October 2016
Accepted:
10 November 2016
Published:
10 January 2017
Abstract: The aim of the present study was to develop and validate HPLC method for determination of related substances of benzydamine hydrochloride in a dosage form, containing benzydamine hydrochloride as an active substance. A new selective, specific and sensitive method was developed for determination of benzydamine degradation products in the formulation. Determination was carried out by reversed-phase HPLC using isocratic solvent elution. The method was validated and found to be precise, accurate and specific; the detector response was linear over 0,05% - 1.2% relative to the nominal concentration of benzydamine hydrochloride in the formulation. The developed HPLC method is useful for determination of benzydamine hydrochloride related substances in formulations and can be applied in Quality Control Laboratories.
Abstract: The aim of the present study was to develop and validate HPLC method for determination of related substances of benzydamine hydrochloride in a dosage form, containing benzydamine hydrochloride as an active substance. A new selective, specific and sensitive method was developed for determination of benzydamine degradation products in the formulation...
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Validation of Soothing Compounding Mixture Technological Process Preparation
Lesia Savchenko,
Victoriya Georgiyants
Issue:
Volume 2, Issue 6, December 2016
Pages:
74-78
Received:
31 October 2016
Accepted:
26 November 2016
Published:
11 January 2017
Abstract: Validation of soothing compounding mixturetechnological process preparation in accordance with the requirements of the quality system organization was done. It is preparingserially in many pharmacies of Ukraine. Obtained results indicate conformity of the finished medicine quality with the regulatory framework requirements of Ukraine in regard to the content of all mixture components.
Abstract: Validation of soothing compounding mixturetechnological process preparation in accordance with the requirements of the quality system organization was done. It is preparingserially in many pharmacies of Ukraine. Obtained results indicate conformity of the finished medicine quality with the regulatory framework requirements of Ukraine in regard to t...
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