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Resveratrol: A Cell Growth Inhibitor Against Leishmania Tropica

Received: 8 December 2023     Accepted: 6 January 2024     Published: 23 January 2024
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Abstract

This study describes cell growth inhibition through invitro, invivo antileishmanial and cytotoxic effect of Resveratrol. Resveratrol was tested at range of concentration of 1 - 10 µg/ml for invitro analysis on L-tropica strain for 24 – 72 h, whereas Sodium Stibogluconate was reference drug. BALB/c mice were subjected to invivo test relative to negative control, while mammalian cells (Lymphocytes) were used in cytotoxic test. After 72 h, tested compound showed significant results against promastigotes at concentration of 1 µg/ml, 2.5 µg/ml, 5 µg/ml, 7.5 µg/ml and 10 µg/ml which were 90.5 ± 0.02, 95.2 ± 0.07, 96.5 ± 0.05, 97.8 ± 0.03 and 98.2 ± 0.04 respectively. After 6 weeks, it showed promising results against intracellular amastigotes in mean lesion size which decrease from 0.9 ± 0.2 mm to 0.25 ± 0.2 mm (p < 0.02) where as 10 mg/kg (dose) was subjected to BALB/c mice which exhibited decrease in percentage cure rate up to 94.01 (95 % C.I = 92.01 – 96.21). IC50 values of lymphocytes showed 14.23 µg/ml (95 % C.I = 10.05 – 17.56) against Resveratrol. After analysis, it was observed that Resveratrol possesses anti-leishmanial activities against the cell growth of L. tropica and has no effect on lymphocytes.

Published in Journal of Drug Design and Medicinal Chemistry (Volume 10, Issue 1)
DOI 10.11648/j.jddmc.20241001.12
Page(s) 8-10
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Resveratrol, Leishmania tropica, Promastigotes, Amastigotes, Mammalian Cell

References
[1] Mazimba, O., Majinda, R. R., Motihanka, D. (2011). Antioxidant and antibacterial constituents from Morus Nigra. Afr. J. Pharm. Pharmacol. 5(6): 751-754.
[2] Iqbal, K., Iqbal, J., Afreen, M. S. (2016b). Comparative Study on Antileishmanial and Cytotoxic activity of Lawsonia Inermis bark and Aloe Vera leaves. Int. J. Biol. Pharm. Allied. Sci. 5(6): 1490-1500.
[3] National Research Council of The National Academy of Sciencies. (2010). Guide for the Care and Use of Laboratory Animals: Eight Edition. Washington, D. C.: The National Academies Press.
[4] Iqbal, K., Iqbal, J., Umair, M., Farooq, U., Iqbal, M. M., Qamar, S., et al. (2016a). Anti-leishmanial and cytotoxic activities of extracts from three Pakistani Plants. Trop. J. Pharm. Res. 15, 2113-2119.
[5] World Health Organization (WHO). (2010). Control of Leishmaniases: Report of the Meeting of the WHO Expert Committee on the Control of Leishmaniases. WHO, Geneva, Switzerland.
[6] Iqbal, K., Jamal, Q., Iqbal, J., Afreen, M. S., Sandhu, M. Z. A., Dar, E., et al. (2017a). Luteolin as a potent Antileishmanial agent against intracellular Leishmania tropica parasites. Trop. J. Pharm. Res. 16(2): 337-342.
[7] Iqbal, K., Iqbal, J., Staerk, D., and Kongstad, K. T. (2017). Characterization of Antileishmanial compounds from Lawsonia Inermis L. Leaves using Semi-high Resolution Antileishmanial Profiling combined with HPLC-HRMS-SPE-NMR. Front. Pharmacol. 2017b; 8: 337.
[8] Hu, H. B., Liang, H. P., Li, H. M., Yuan, R. N., Sun, J., Zhang, L. L. et al., (2018). Isolation, modification and cytotoxic evaluation of Resveratrols from Acanthopanax leucorrhizus. Fitoterapia. 124: 167-176. doi: 10.1016/j.fitote.2017.11.007.
[9] Ghada M. Abbas, Fatma M. Abdel Bar, Hany N. Baraka, Ahmed A. Gohar & Mohammed-Farid Lahloub (2014) A new antioxidant stilbene and other constituents from the stem bark of Morus nigra L., Natural Product Research: Formerly Natural Product Letters, 28: 13, 952-959, DOI: 10.1080/14786419.2014.900770.
[10] Antinarelli, L. M. R., Meinel, R. S., Coelho, E. A. F., Silva, A. D., & Coimbra, E. S. (2019). Resveratrol analogues present effective antileishmanial activity against promastigotes and amastigotes from distinct Leishmania species by multitarget action in the parasites. Journal of Pharmacy and Pharmacology. doi: 10.1111/jphp.13177.
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  • APA Style

    Iqbal, K., Aziz, Q., Sana, A. (2024). Resveratrol: A Cell Growth Inhibitor Against Leishmania Tropica. Journal of Drug Design and Medicinal Chemistry, 10(1), 8-10. https://doi.org/10.11648/j.jddmc.20241001.12

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    ACS Style

    Iqbal, K.; Aziz, Q.; Sana, A. Resveratrol: A Cell Growth Inhibitor Against Leishmania Tropica. J. Drug Des. Med. Chem. 2024, 10(1), 8-10. doi: 10.11648/j.jddmc.20241001.12

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    AMA Style

    Iqbal K, Aziz Q, Sana A. Resveratrol: A Cell Growth Inhibitor Against Leishmania Tropica. J Drug Des Med Chem. 2024;10(1):8-10. doi: 10.11648/j.jddmc.20241001.12

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  • @article{10.11648/j.jddmc.20241001.12,
      author = {Kashif Iqbal and Qaiser Aziz and Ayesha Sana},
      title = {Resveratrol: A Cell Growth Inhibitor Against Leishmania Tropica},
      journal = {Journal of Drug Design and Medicinal Chemistry},
      volume = {10},
      number = {1},
      pages = {8-10},
      doi = {10.11648/j.jddmc.20241001.12},
      url = {https://doi.org/10.11648/j.jddmc.20241001.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20241001.12},
      abstract = {This study describes cell growth inhibition through invitro, invivo antileishmanial and cytotoxic effect of Resveratrol. Resveratrol was tested at range of concentration of 1 - 10 µg/ml for invitro analysis on L-tropica strain for 24 – 72 h, whereas Sodium Stibogluconate was reference drug. BALB/c mice were subjected to invivo test relative to negative control, while mammalian cells (Lymphocytes) were used in cytotoxic test. After 72 h, tested compound showed significant results against promastigotes at concentration of 1 µg/ml, 2.5 µg/ml, 5 µg/ml, 7.5 µg/ml and 10 µg/ml which were 90.5 ± 0.02, 95.2 ± 0.07, 96.5 ± 0.05, 97.8 ± 0.03 and 98.2 ± 0.04 respectively. After 6 weeks, it showed promising results against intracellular amastigotes in mean lesion size which decrease from 0.9 ± 0.2 mm to 0.25 ± 0.2 mm (p 50 values of lymphocytes showed 14.23 µg/ml (95 % C.I = 10.05 – 17.56) against Resveratrol. After analysis, it was observed that Resveratrol possesses anti-leishmanial activities against the cell growth of L. tropica and has no effect on lymphocytes.},
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Resveratrol: A Cell Growth Inhibitor Against Leishmania Tropica
    AU  - Kashif Iqbal
    AU  - Qaiser Aziz
    AU  - Ayesha Sana
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    DO  - 10.11648/j.jddmc.20241001.12
    T2  - Journal of Drug Design and Medicinal Chemistry
    JF  - Journal of Drug Design and Medicinal Chemistry
    JO  - Journal of Drug Design and Medicinal Chemistry
    SP  - 8
    EP  - 10
    PB  - Science Publishing Group
    SN  - 2472-3576
    UR  - https://doi.org/10.11648/j.jddmc.20241001.12
    AB  - This study describes cell growth inhibition through invitro, invivo antileishmanial and cytotoxic effect of Resveratrol. Resveratrol was tested at range of concentration of 1 - 10 µg/ml for invitro analysis on L-tropica strain for 24 – 72 h, whereas Sodium Stibogluconate was reference drug. BALB/c mice were subjected to invivo test relative to negative control, while mammalian cells (Lymphocytes) were used in cytotoxic test. After 72 h, tested compound showed significant results against promastigotes at concentration of 1 µg/ml, 2.5 µg/ml, 5 µg/ml, 7.5 µg/ml and 10 µg/ml which were 90.5 ± 0.02, 95.2 ± 0.07, 96.5 ± 0.05, 97.8 ± 0.03 and 98.2 ± 0.04 respectively. After 6 weeks, it showed promising results against intracellular amastigotes in mean lesion size which decrease from 0.9 ± 0.2 mm to 0.25 ± 0.2 mm (p 50 values of lymphocytes showed 14.23 µg/ml (95 % C.I = 10.05 – 17.56) against Resveratrol. After analysis, it was observed that Resveratrol possesses anti-leishmanial activities against the cell growth of L. tropica and has no effect on lymphocytes.
    VL  - 10
    IS  - 1
    ER  - 

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Author Information
  • Department of Pharmacy, IBADAT International University, Islamabad, Pakistan

  • Department of Pharmacy, IBADAT International University, Islamabad, Pakistan

  • Department of Pharmacy, IBADAT International University, Islamabad, Pakistan

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